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Abstract #4019

Differentiation of Cervical Tubercular with Non-Reactive Lymphadenitis Tissues by Proton HR-MAS NMR Spectroscopy

Raja Roy1, Shatakshi Srivastava1, Kushagra Gaurav2, Surender Kumar2, Madhu M. Goel, Rajiv Garg, Abhinav A. Sonkar2

1Centre of Biomedical Magnetic Resonance, Lucknow, Uttar Pradesh, India; 2General Surgery, King Georges Medical University, Lucknow, Uttar Pradesh, India

In the present work, proton HR-MAS NMR spectroscopic studies have been performed on human cervical lymph nodes incisional biopsy tissues specimens, obtained from 109 patients, for the identification of metabolic fingerprints in cervical tubercular lymphadenitis. Thirty metabolites were identified. Significant decrease in myo-inositol and increase in amino acids were observed in the spectra of tubercular tissues. The proton NMR spectra were then subjected to PCA and PLS-DA multivariate analysis. The validated model allowed >98% correct classification of tubercular from non-reactive tissues when compared with gold standard histopathological examination