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Abstract #4018

Metabolomics of Breast Cancer Serum Using 1H Nuclear Magnetic Resonance Spectroscopy

Ashish Gupta1, Pooja Ramakant2, Pushplata Sankhwar3, Navneeta Bansal4

1Centre of Biomedical Magnetic Resonance, SGPGIMS Campus, Lucknow, UP, India; 2Endocrine and Breast Surgery, Department of Surgery, Christian Medical College,, Vellore, Tamil Nadu, India; 3Department of Obstetrics & Gynecology, King Georges Medical University, Lucknow, UP, India; 4Department of Biochemistry, King Georges Medical University, Lucknow, UP, India


A novel combining approach of 1H NMR spectroscopy of serum metabolite profile and linear multivariate discriminant function analysis (DFA) was carried out to identify the differential biomarkers of breast cancer (BC). The study was conducted on 60 healthy women and women with breast disorders-mainly malignant (n = 60) and fibroadenoma and cystosarcoma phylloides (n = 65). DFA reveals that 1H NMR measured metabolites can differentiate (99.9%) not only between healthy controls and breast disordered samples but also malignant and non-malignant breast tumors (99%). This novel approach may serve as a rapid and promising surrogate diagnostic probe for screening breast disorders.

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