Robin A. de Graaf1, Cosimo Giannini1, Sonia Caprio1, Raimund I. Herzog1
1Yale University, MRRC, New Haven, CT, United States
1H NMR is a popular technique to detect several dozen metabolites in blood plasma. However, in order to become a quantitative and high-throughput method 1H NMR has to overcome several challenges, related to a dominant lipoprotein baseline and automated and objective spectral quantification. Here we present an approach that measures the lipoprotein signals separate from metabolite signals based on the large difference in diffusion. After the lipoprotein spectrum has been parameterized, it can be used as part of a spectral basis set in the subsequent quantification of the total (metabolites + lipoprotein) 1H NMR spectrum.