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Abstract #4029

Quantification of Phosphoenolpyruvate in the Human Liver and Its Application in a Meal Study Employing 31P MRS

Alessandra Laufs1, Roshan Livingstone2, Maria Fritsch3, Julia Szendroedi4, Juergen Bunke5, Michael Roden1, 6, Jong-Hee Hwang1

1Institute for Clinical Diabetology, German Diabetes Center, Dsseldorf, Germany; 2Department of Radiology, Christian Medical College, Vellore, India; 3Medical University of Vienna, Vienna, Austria; 4Institute for Energy Metabolism, German Diabetes Center, Dsseldorf, Germany; 5Philips Healthcare, Hamburg, Germany; 6Department of Metabolic Deseases, University Clinics Dsseldorf, Dsseldorf, Germany

Changes in phosphoenolpyruvate (PEP) concentrations of the liver were quantified before and after a high-carbohydrate meal using 31P MRS in six healthy subjects at 3T. Quantification of PEP was performed using matching phantoms and an external reference in order to correct for excitation pulse profile, coil loading, B1 field inhomogeneity, T1 relaxation time (T1 = 0.82 0.16 s, separately assessed, N = 5)and the amount of liver fat. The concentration of PEP 140 min after the meal was significantly decreased from 1.14 0.22 mmol/l to 0.86 0.22 mmol/l (N = 6, p = 0.01, paired t-test).