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Abstract #4226

Amine/Amide Concentration Independent Detection (AACID) of Intracellular pH by CEST MRI at 9.4T

Nevin McVicar1, Alex Li2, Robert Bartha2, Daniela F. Goncalves3, 4, Susan Meakin2, Marco A. Prado2

1Medical Biophysics, University of Western Ontario (UWO), London, ON, Canada; 2Robarts Research Institute, London, ON, Canada; 3Physiology and Pharmacology, UWO, London, ON, Canada; 4Graduate Program in Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

A novel ratiometric CEST approach is developed for amine/amide concentration independent detection (AACID) of intracellular pH. In vitro results demonstrate a linear relation between pH and the amine/amide CEST ratio. In vivo pH-calibration allowed quantitative pH mapping in both stroke and brain tumor mouse models.