Ex Vivo 1H Magnetic Resonance Spectroscopy of Intact Salmon Muscle Via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence Technique

Honghao Cai¹, Xiaohong Cui¹, Shuhui Cai¹, Zhong Chen¹

The flavour and nutrition of a fish are greatly influenced by its fat content and its composition. However, it is difficult to obtain high-resolution (HR) NMR spectrum of intact fish tissues by traditional methods owing to the magnetic susceptibility gradients among the tissues. In this study, a Hadamard-encoded intermolecular multiple-quantum coherence (iMQC) method was implemented to obtain HR NMR spectrum of intact salmon muscle. The experimental results indicate that iMQC technique is a feasible way for HR NMR spectrum of fish tissues. Compared to magic angle spinning, it is non-invasive and suitable for in vivo and in situ applications.