Identifying Intracellular Sperm Metabolites Using HR MAS NMR
Jack Tristan Pearson 1 , Steven Reynolds 2 , Adriana Bucur 2 , Allan Pacey 1 , and Martyn Paley 2
Human Metabolism, The University of
Sheffield, Sheffield, South Yorkshire, United Kingdom,
Science, The University of Sheffield, Sheffield, South
Yorkshire, United Kingdom
Approximately 25% of males have poor semen quality and
diagnosis of male infertility relies on techniques
developed in the 1950s. To investigate sperm
metabolism, HR MAS NMR of boar sperm was performed at
two incubation temperatures and in the presence or
absence of α-chlorohydrin (an inhibitor of glycolysis).
HR MAS successfully identified significant increases in
acetate, glutamine and glucose at increased incubation
temperatures. A significant decrease in lactate was
identified when metabolism was inhibited with α-chlorohydrin.
This data supports the potential use for HR MAS in
identification of metabolic biomarkers in sperm and for
infertility diagnosis in the clinical setting.
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