Abstract #0848
13C MRS detects TCA down-regulation in mutant IDH1 glioma cells
Jose Luis Izquierdo Garcia 1 , Pia Eriksson 1 , Cai Larry 1 , Myriam Chaumeil 1 , Russell O Pieper 2 , Joanna J Phillips 2 , and Sabrina M Ronen 1
1
Radiology, UCSF, San Francisco, CA, United
States,
2
Neurological
Surgery, Helen Diller Research Center, UCSF, San
Francisco, CA, United States
Mutations in Isocitrate dehydrogenase (IDH1) have been
reported in over 70% of low-grade gliomas and secondary
glioblastomas. In this study, the metabolism of live
U87IDHmut and U87IDHwt cells was probed by thermally and
Hyper-polarized (HP) 13C-MRS. HP-1,2-13C Piruvic Acid
MRS showed a drop in 5-13C glutamate production and an
increase in lactate and alanine production. 1-13C
glucose infusion resulted in a drop of glutamate in
U87IDHmut cells whereas glucose uptake was not
significant difference. The increase in lactate and
alanine and the decrease of glutamate production in
U87IDHmut cells indicate that TCA down-regulation is a
major effect of IDH mutation.
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