Abstract #0847
2-Hydroxyglutarate Labeling from [U-13C]glucose in Human Glioma Cells
Hyeon-Man Baek 1,2 , Yun-Ju Lee 1 , Gregory Hyung Jin Park 1 , Eun-Hee Kim 1 , and Chaejoon Cheong 1,2
1
Division of MR Research, Korea Basic Science
Institute, Ochang, Chungbuk, Korea,
2
Department
of Bio-Analytical Science, University of Science &
Technology, Yuseong-gu, Daejeon, Korea
We present here that the data analysis of 1H- and
13C-NMR spectra of the cell extracts showed a
significant increase in the concentration of the 2HG in
IDH mutated cells, but not in IDH wild type cells and
mutant IDH cells with low transfection efficiency. In
particular, 2HG-C5 labeling peaks from 13C-substrate
were well separated from other metabolites (e.g.,
glutamate C-5 at 182.04, lactate C-1 at 183.23 ppm,
etc.) in the 900MHz 1H-NMR spectra. This result
demonstrates that 2HG can be actively being produced
during the 24h period of [U-13C]glucose substrate
feeding.
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