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Abstract #1002

HP- 13 C-NMR detects the alternation of LDH kinetics and redox state induced by metabolite modulation

Lin Z. Li 1,2 , Stephen Kadlececk 1 , Hoora Shaghaghi 1 , He N. Xu 1,2 , Harrilla Profka 1 , and Rahim Rizi 1

1 Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States, 2 Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania, United States

Abnormal enzyme activities and cellular redox state have been observed in various medical conditions including cancer, diabetes, neurodegenerative diseases and lung diseases. We employed hyperpolarized 13C-pyruvate NMR to investigate non-invasively how the enzyme kinetics (rate constants) and cellular redox state may be modulated by the levels of metabolites. Our results indicate a strong coupling of the lactate dehydrogenase reaction with the cellular redox state. Hyperpolarized 13C-pyruvate can be a metabolic indicator of redox state for the study of normal physiology and diseases.

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