Abstract #1082
Metabolic profile of human breast tissues studied by in-vitro NMR spectroscopy
Naranamangalam R Jagannathan 1 , Rani G Sah 1 , Uma Sharma 1 , Rajinder Parshad 2 , and Vurthaluru Seenu 2
1
Department of NMR & MRI Facility, All India
Institute of Medical Sciences, New Delhi, Delhi, India,
2
Department
of Surgical Disciplines, All India Institute of Medical
Sciences, New Delhi, Delhi, India
High-resolution in-vitro NMR spectroscopy was used to
determine the absolute concentration of various
metabolites in 23 breast tissues, both involved (n=11)
and non-involved (n=13). One-dimensional and two-
dimensional NMR experiments (DQF-COSY and TOCSY) were
carried out at 400.13 MHz. The concentration of Lactate
(14.0 11.8 mmol/kg), Creatine (1.9 1.2 mmol/kg),
Choline (3.1 2.2 mmol/kg), Glycerophosphocholine/Phosphocholine
(5.2 4.1 mmol/kg) and Glutamate/Glutamine (8.3 5.4)
mmol/kg in involved tissues were significantly higher
compared to non-involved tissues, indicating a general
increase in the metabolic activity in tumor tissues and
thus providing an insight into the tumor metabolism.
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