Abstract #2775
Measurements and Modeling of Hyperpolarized 1-13C in the Perfused Lung in Relation to Organ Redox State
Stephen J. Kadlecek 1 , Hoora Shaghaghi 1 , Mehrdad Pourfathi 1 , Sarmad Siddiqui 1 , Jennia Rajaei 1 , Profka Harrilla 1 , and Rahim R. Rizi 1
1
Radiology, University of Pennsylvania,
Philadelphia, Pennsylvania, United States
We present the measurements of lactate pool labeling
rates in the isolated, perfused rat lung using
hyperpolarized 1-13C pyruvate. The rates are shown to
vary strongly with perfusate constituents (unpolarized
lactate and pyruvate, as well as hyperpolarized pyruvate
concentration and can be accurately reproduced using a
three-compartment model which incorporates previously
measured transport and enzymatic activity measurements,
as well as concentrations of enzyme and cofactors. We
conclude that intracellular conditions (e.g., redox
state) are strongly perturbed during a typical
hyperpolarized pyruvate measurement, and that
consideration of metabolic flux through LDH is not
necessary to reproduce experimental results.
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