Abstract #2785
Metabolism of Hyperpolarized [1- 13 C]pyruvate to Plasma Glucose in the Rat Liver
Karlos X Moreno 1 , Eunsook Jin 1 , Jian-Xiong Wang 1 , A Dean Sherry 1 , Matthew E Merritt 1 , and Craig R Malloy 1
1
Advanced Imaging Research Center, University
of Texas Southwestern Medical Center, Dallas, Texas,
United States
In vivo hepatic metabolism of hyperpolarized
[1-13C]pyruvate is not known to produce 13C-enriched
blood glucose. Rats were either infused simultaneously
with [3-13C]pyruvate and [U-13C]octanoate or saline
before injection with hyperpolarized [1-13C]pyruvate
Hyperpolarized [13C]bicarbonate was observed in several
animals. Carbon-13 isotopomer analysis of purified blood
glucose showed a large amount of 13C enrichment from
pyruvate/octanoate infused animals. Saline infused
animals showed a small amount of 13C-enrichment. Also
evident were spin-spin 13C couplings was observed as
being derived from two molecules of [1-13C]pyruvate
forming one molecule of glucose. Integration of
hyperpolarized and conventional 13C-NMR is a powerful
method for monitoring liver metabolism.
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