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Abstract #2798

Non-invasive measurement of cellular membrane pH gradient in brain tumors using hyperpolarized 13 C-bicarbonate MSRI and CEST imaging

Heeseung Lim 1 , Nevin McVicar 1 , Francisco M Martinez-Santiesteban 1 , Robert Bartha 1,2 , and Timothy J Scholl 1,2

1 Medical Biophysics, Western University, London, Ontario, Canada, 2 Robarts Research Institute, Western University, London, Ontario, Canada

The pH gradient between intracellular and extracellular space changes in tumor. Tumors have a more alkaline pHi and a more acidic pHe. In the current study, pHi and pHe spatial distribution maps are measured in a glioma brain tumor rat model using CEST 1 H-MRI and hyperpolarized 13 C-MRS respectively. Measured pHi and pHe in normal and tumor tissue are consistent with previous work. The pH maps demonstrate an increased transmembrane pH gradient, ∆pH ≈ 0.14 in the glioma compared to contralateral brain tissue, ∆pH ≈ -0.34. This work represents to the authors best knowledge the first in vivo quantitative pH-gradient mapping.

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