Abstract #2800
A new low volume NMR tube for in-vitro hyperpolarized 13 C experiments of prostate cancer cell suspensions
Vincent Breukels 1 , Cees F.J. Jansen 2 , Jack J.A. van Asten 1 , Jan W.M. van Os 3 , Andrea Capozzi 4 , P. Jan M. van Bentum 3 , Arnaud Comment 4 , and Tom W.J. Scheenen 1
1
Radiology, Radboud University Medical
Center, Nijmegen, Gelderland, Netherlands,
2
Urology,
Radboud University Medical Center, Nijmegen, Gelderland,
Netherlands,
3
Institute
for Molecules and Materials, Radboud University,
Nijmegen, Gelderland, Netherlands,
4
Laboratory
for Functional and Metabolic Imaging, cole
Polytechnique Fdrale de Lausanne, Lausanne,
Switzerland
In-vitro
experiments
of hyperpolarized
13
C
substrates is a valuable tool to study normal and
pathogenic metabolic processes. Commonly these
experiments are performed in cell suspensions containing
between 25 100 million cells. We developed a low
volume, low number of cells setup. Using two prostate
cancer cell lines we show that accurate quantification
of metabolic conversion and perfect resolution (< 1Hz
linewidth) can be obtained with as little as two million
cells. Our setup expands the use of hyperpolarized MR
beyond immortalized cell lines, such as primary cell
cultures, for which high number of cells cannot be
obtained.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Click here for more information on becoming a member.
