Abstract #2803
Tumor-specific expression and detection of a CEST reporter gene
Il Minn 1 , Amnon Bar-Shir 1,2 , Jeff W. M. Bulte 1,2 , Paul B. Fisher 3 , Assaf A. Gilad 1,2 , and Martin G. Pomper 1
1
The Russell H. Morgan Department of
Radiology and Radiological Science, Johns Hopkins
University, Baltimore, MD, United States,
2
Cellular
Imaging Section, Institute for Cell Engineering, Johns
Hopkins University, Baltimore, MD, United States,
3
Massey
Cancer Center, Virginia Commonwealth University,
Richmond, VA, United States
We developed a molecular-genetic imaging approach to
accomplish tumor-specific expression of a reporter gene
based on chemical exchange saturation transfer (CEST)
MRI. The lysine-rich protein (LRP), a CEST reporter, was
expressed under the control of the PEG-Prom, which is
tumor-specific, in 9L rat glioma cells (9L
PEG-LRP
).
9L
PEG-LRP
cells
transplanted to a mouse brain showed higher CEST
contrast compared to those from wild type cells. Here we
show the feasibility of using a CEST-reporter gene under
a tumor-specific promoter. This is the first step toward
a systemic delivery of a PEG-Prom:LRP vector, which
allows visualization of malignant tissues with CEST MRI.
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