Abstract #2804
MRI Tracking of Transplanted Cell Viability and Function Using a Multimodal Quadruple Fusion Gene Reporter
Anna V Naumova 1,2 , Nathan J Palpant 2,3 , Niranjan Balu 1,2 , Xia Shen 1,4 , Chun Yuan 1,5 , and Charles E Murry 2,3
1
Radiology, University of Washington,
Seattle, WA, United States,
2
Center
for Cardiovascular Biology, University of Washington,
Seattle, WA, United States,
3
Pathology,
University of Washington, Seattle, WA, United States,
4
Medicine,
University of Washington, Seattle, WA, United States,
5
Bioengineering,
University of Washington, Seattle, WA, United States
We propose an integrative approach to monitor
transplanted cells non-invasively by combining MRI based
structural and functional evaluation with other imaging
modalities such as bioluminescence imaging, fluorescence
and PET. For this, we engineered a novel
genetically-based sensing system consisting of ferritin,
luciferase, GCaMP3, and HSV1-tk, which makes graft
detectable by multiple imaging modalities. Human
embryonic stem cell line expressing quadruple gene
reporter demonstrated T2 shortening effect in MRI,
strong bioluminescence and fluorescence properties as
well as ganciclovir sensitivity. This integrative
approach enables longitudinal non-invasive monitoring of
the transplanted cells.
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