Abstract #2812
PHENOTYPIC AND FUNCTIONAL ASSESSMENT OF MAGNETICALLY LABELED PIG EMBRYONIC STEM CELL DERIVED HEPATOCYTES
Neil C Talbot 1 , Paul Graninger 1 , Dwayne Roach 1,2 , Erik M Shapiro 2 , Wesley M Garrett 1 , and Thomas J Caperna 1
1
Beltsville Agricultural Research Center,
USDA, Beltsville, MD, United States,
2
Department
of Radiology, Michigan State University, East Lansing,
MI, United States
Many severe, progressive liver diseases may be amenable
to hepatocyte transplantation. PICM-19 cells are
bipotent liver parenchymal cells derived from pig ESCs
and are promising for swine regenerative medicine models
of liver disease and for potential human
xenotransplantation. Here we demonstrate that magnetic
labeling of PICM-19 hepatocytes with MPIOs does not
impact the phenotypic or functional properties of these
cells, paving the way for their use in MRI-based cell
tracking of liver regenerative medicine paradigms.
Further, these experiments demonstrate the usefulness of
functional assays, rather than detection of cell-surface
CD markers for studying effects of magnetic particles on
labeled cells.
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