Abstract #2957
31P MRS Assessment of Hepatic Mitochondrial Toxicity in Rat
Hasan Alsaid 1 , Mary V Rambo 1 , Tinamarie Skedzielewski 1 , James R Armitage 2 , Jon J Lyon 2 , and Beat M Jucker 1
1
Preclinical & Translational Imaging, LAS,
PTS, GlaxoSmithKline, King of Prussia, PA, United
States,
2
Safety
Assessment Tox/Path/Investigative, PTS, GlaxoSmithKline,
Ware, Hertfordshire, United Kingdom
A clinically translatable 31P-MRS method was used to
assess the high energy metabolite profile after the
administration of GSK121A (50mg/kg or 25mg/kg), a known
mitochondrial respiratory chain inhibitor. The results
showed that liver ATP concentrations remained unchanged,
Pi and Pi/ATP ratio were increased significantly in the
GSK121A treated groups compared to vehicle, associated
with a higher blood lactate level which correlated with
the change in body temperature. While steady state ATP
levels appear to be normal, there could potentially be a
decrease in ATP synthesis associated with decreased body
temperature and increase in Pi. This method may be used
to assess drug effects on hepatic mitochondrial function
in patients.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Click here for more information on becoming a member.
