Meeting Banner
Abstract #2971

Exploring Energy Metabolism in Zebrafish Brain: A 1 H-[ 13 C]-NMR Study

Anant Bahadur Patel 1 , Madhavi Adusmilli 1 , Sandeep Saxena 1 , and Mohammed M Idris 1

1 CSIR-Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India

Brain metabolism was investigated in zebrafish by 1 H-[ 13 C]-NMR spectroscopy. Zebrafish were anesthetized with tricane and [1,6- 13 C 2 ]glucose was administered through intracardiac injection. The concentration and 13 C labeling of brain metabolites were measured using 1 H-[ 13 C]-NMR spectroscopy in brain extract. The 13 C turnover of brain amino acids from [1,6- 13 C 2 ]glucose in zebrafish is similar to the pattern observed in rat and mouse brain. Moreover, the brain energy metabolism in zebrafish is decreased with acute ethanol treatment. Zebrafish would be useful alternate model to study mechanism of various neurological disorders and screening of drugs.

This abstract and the presentation materials are available to members only; a login is required.

Join Here