Exploring Energy Metabolism in Zebrafish Brain: A 1 H-[ 13 C]-NMR Study

Brain metabolism was investigated in zebrafish by ¹ H-[ ¹³ C]-NMR spectroscopy. Zebrafish were anesthetized with tricane and [1,6- ¹³ C ₂ ]glucose was administered through intracardiac injection. The concentration and ¹³ C labeling of brain metabolites were measured using ¹ H-[ ¹³ C]-NMR spectroscopy in brain extract. The ¹³ C turnover of brain amino acids from [1,6- ¹³ C ₂ ]glucose in zebrafish is similar to the pattern observed in rat and mouse brain. Moreover, the brain energy metabolism in zebrafish is decreased with acute ethanol treatment. Zebrafish would be useful alternate model to study mechanism of various neurological disorders and screening of drugs.

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