Meeting Banner
Abstract #3043

Two hour-sustained brain activation in the anesthetized rat

Sarah Sonnay 1 , Joo M.N. Duarte 1,2 , Rolf Gruetter 1,3 , and Nathalie Just 1,2

1 LIFMET, EPFL, Lausanne, Switzerland, 2 Radiology, University of Lausanne, Lausanne, Switzerland, 3 Radiology, Universities of Geneva and Lausanne, Geneva/Lausanne, Switzerland

Brain circuitry can be studied with BOLD fMRI, but long stimulation paradigms might be needed to investigate long-term neuronal modifications. The aim of the study was to develop a method to activate the rat brain for 2 hours. SD rats (n=10,32213g) under α-chloralose anesthesia underwent electrical stimulation of the forepaw. BOLD fMRI was performed at 9.4T using GE-EPI. We observed sustained and localized BOLD responses with minimum activated volume loss during 2 hour-stimulation in varying the frequency at regular intervals and in using small ISI. We conclude that the applied paradigm increases the statistical power for connectivity analysis.

This abstract and the presentation materials are available to members only; a login is required.

Join Here