Diffusion-weighted HR-MAS of biopsies to obtain separated fat-free metabolite and lipid spectra. A feasibility study.
Galle Diserens 1 , Martina Vermathen 2 , Christina Stahl 3 , Nicholas T. Broskey 4 , Chris Boesch 1 , Francesca Amati 1,4 , and Peter Vermathen 1
Depts Clinical Research and Radiology,
University Bern, Bern, Bern, Switzerland,
of Chemistry & Biochemistry, University Bern, Bern,
of Clinical Veterinary Medicine, Clinical Radiology,
Vetsuisse-Faculty, University Bern, Bern, Switzerland,
of Physiology, University of Lausanne, Lausanne,
HR-MAS NMR diffusion measurements with strong and low
diffusion weighting were used to obtain fat-free
spectra: Pure lipid spectra obtained at strong diffusion
weighting were subtracted from those obtained at low
diffusion weighting, which include both, small
metabolites and lipids. This operation results in a
spectrum with low lipid contamination under specific
metabolite peaks, allowing for a more accurate
quantification. This technique has therefore potential
to improve chemometric analysis for biomarkers
detection. Moreover, the diffusion scans permit a
separate lipid analysis and a regular diffusion analysis
without expanding scan time.
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