Abstract #3753
Towards MRS using High-Resolution Magic-Angle Coil Spinning: application to brain metabolism
Alan Wong 1 , Beatriz Jimnez 2 , Grard Raffard 3 , Jean-Michel Franconi 3 , and Anne-Karine Bouzier-Sore 3
1
SIS2M/LSDRM, CEA Saclay/UMR3299,
Gif-sur-Yvette, France,
2
Department
of Surgery and Cancer, Clinical Phenotyping Centre,
London, United Kingdom,
3
CRMSB/UMR5536,
CNRS/Universit Bordeaux Segalen, Bordeaux, France
High-Resolution Magic-Angle sample Spinning (HR-MAS) NMR
spectroscopy of biopsies combined with chemometric
statistical tools has now emerged as a powerful
methodology for metabolomics NMR and has led to many
important disease diagnosis, therapeutic target
discovery and environmental assessment. This technique
is also a method of choice when studying metabolism.
However, due to the intrinsically poor detection
sensitivity, NMR analysis often requires large tissue
mass (5 to 10 mg). Such mass could compromise the
metabolic evaluation due to the high degree of tissue
heterogeneity (in tumor for example). Unfortunately,
today there are no practical means for NMR analysis of
small quantity of tissue, or any soft-matter, where
sample magic-angle spinning is essential for high
quality data acquisition. For this reason, we are
developing NMR-based analytical tools with good
sensitivity and with good metabolic spectral quality for
nanogram tissue biopsies1. Currently, one promising
approach is the used of a simple micro-resonator
(High-Resolution Magic-Angle Coil Spinning (MACS)), in
which it can wirelessly coupled to a MAS probe. Here, we
present the use of this micro-resonator High-Resolution
(HR)MACS to explore rat brain metabolism, and to see if
it was possible to detect and discriminate any
differential biomarkers between rest and activated rat
brain.
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