High-resolution 1 H magnetic resonance spectroscopy of whole fish, fish eggs and fish muscles via intermolecular multiple-quantum coherence

Extraction and high-resolution magic angle spinning (HR MAS) are widely applied for HR NMR spectra of tissues. However, both of the methods are subject to limitations. In this study, the feasibility of ¹ H ex vivo and in vivo NMR spectroscopy based on Hadamard-encoded intermolecular multiple-quantum coherence (iMQC) technique is explored using fish muscle, fish eggs and a whole fish as examples. Experimental results indicate that HR in vivo NMR spectra of these samples can be obtained by the iMQC technique. Hadamard encoding improves acquisition efficiency. Compared to MAS, it is non-invasive and suitable for in vivo and in situ applications.

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