Abstract #4433
In-Bore Oxygenator Designed for Physiological Maintenance of Slice and Cell Culture Preparations Employed in MR Microscopy Studies
Jeremy J Flint 1,2 , Kannan M Menon 2,3 , Brian Hansen 4 , and Stephen J Blackband 1,5
1
Neuroscience, University of Florida,
Gainesville, Florida, United States,
2
McKnight
Brain Institute, University of Florida, Gainesville,
Florida, United States,
3
Biomedical
Engineering, University of Florida, Gainesville,
Florida, United States,
4
Center
for Functionally Integrative Neuroscience, Aarhus
University, Aarhus, Denmark,
5
National
High Magnetic Field Laboratory, Florida State
University, Tallahassee, Florida, United States
Recent developments allowing direct visualization of
cellular structure in excised mammalian tissue using MRI
techniques have created a need for micro-perfusion
equipment capable of operating in conjunction with
high-field spectrometer hardware. This study details the
design and fabrication of an in-bore oxygenator capable
of offering precise control of perfusate properties
including pH and dissolved gas (O
2
&
CO
2
) content. In addition, a stability
assessment is conducted which compares diffusion signal
reproducibility over time under conditions of constant
perfusion as compared to a static control group.
Findings indicate statistical equivalence at 13 of 14
time-points sampled over a 21 hour period.
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