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Abstract #4433

In-Bore Oxygenator Designed for Physiological Maintenance of Slice and Cell Culture Preparations Employed in MR Microscopy Studies

Jeremy J Flint 1,2 , Kannan M Menon 2,3 , Brian Hansen 4 , and Stephen J Blackband 1,5

1 Neuroscience, University of Florida, Gainesville, Florida, United States, 2 McKnight Brain Institute, University of Florida, Gainesville, Florida, United States, 3 Biomedical Engineering, University of Florida, Gainesville, Florida, United States, 4 Center for Functionally Integrative Neuroscience, Aarhus University, Aarhus, Denmark, 5 National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, United States

Recent developments allowing direct visualization of cellular structure in excised mammalian tissue using MRI techniques have created a need for micro-perfusion equipment capable of operating in conjunction with high-field spectrometer hardware. This study details the design and fabrication of an in-bore oxygenator capable of offering precise control of perfusate properties including pH and dissolved gas (O 2 & CO 2 ) content. In addition, a stability assessment is conducted which compares diffusion signal reproducibility over time under conditions of constant perfusion as compared to a static control group. Findings indicate statistical equivalence at 13 of 14 time-points sampled over a 21 hour period.

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