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Abstract #4661

A new set-up to investigate neurophysiological effects of CO2-induced ocean acidification on the brain of fish via MR imaging and spectroscopy

Matthias Schmidt 1 , Hans-Otto Prtner 1 , and Christian Bock 1

1 Integrative Ecophysiology, Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Bremen, Germany

CO2-induced ocean acidification leads to neurological disorders in fish with unknown consequences for marine ecosystems. In order to investigate the exact mechanisms of how CO2 interferes with the teleost nervous system we developed a stereotactic holding device enabling in vivo MR imaging and spectroscopy on the brain of fish for several days without use of anaesthetics during the experimental procedure. This setup was used to acquire anatomical images of the brain of an Antarctic Notothenoid to identify possible regions for future studies. Following we investigated the effects of severe hypercapnia on the acid-base and energy status.

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