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Abstract #0209

Kinetic Analysis of Dynamic Deuterium MR Spectra for Simultaneous Assessment of Cerebral Glucose Consumption Rate and TCA Cycle Flux

Ming Lu 1 , Xiao-Hong Zhu 1 , and Wei Chen 1

1 Center for Magnetic Resonance Research, University of Minnesota Medical School, Minneapolis, Minnesota, United States

Simultaneous assessment of cerebral glucose consumption rate (CMR glc ) and TCA cycling rate (V TCA ) is crucial for understanding neuroenergetics under various physiopathological conditions; nevertheless, such measurement was not possible. Recently, we developed a novel in vivo Deuterium ( 2 H) MR (DMR) approach for noninvasively assessing glucose metabolisms in rat brain at 16.4 T. Following a brief injection of deuterated glucose, the dynamic labeling on glucose, glutamate/glutamine (Glx), water and lactate in the brain tissue can be monitored via tracking their well-resolved 2 H signals. To quantify metabolic rates, a new kinetic model incorporating glycolysis, TCA cycle and lower case Greek alpha -ketoglutarate/Glx exchange was developed in this study. By least-square fitting of the model with the experimental data obtained from dynamic DMR measurement in rat brains, major metabolic fluxes such as CMR glc and V TCA can be determined concurrently. Two different metabolic conditions were examined; increased CMR glc (0.46 vs. 0.28 mol/g/min) and V TCA (0.96 vs. 0.6 mol/g/min) were found under morphine infusion as compare to the deeper anesthesia of 2% isoflurane, which are consistent with previous in vivo 13 C or 1 H MRS studies. In summary, we demonstrate that the new DMR approach in combine with the kinetic model described in this work is capable of simultaneously determining CMR glc and V TCA with high sensitivity and reliability.

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