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Abstract #0371

Multimodal in vivo evaluation of a surface-switching nanoparticle platform

Francois Fay 1 , Line Hansen 2 , Stephanie J. Hectors 3 , Jun Tang 1 , Anita Gianella 1 , Brenda L. Sanchez-Gaytan 1 , Yiming Zhao 1 , Aneta J. Mieszawska 1 , Robert Langer 4 , Claudia Calcagno 1 , Gustav J. Strijkers 3,5 , Zahi A. Fayad 1 , and Willem J.M. Mulder 1,5

1 Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, New York City, New York, United States, 2 Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark, 3 Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands, 4 Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States, 5 Department of Vascular Medicine, Academic Medical Center, Amsterdam, Netherlands

We have developed a hybrid lipid-polymer nanoparticle platform, which has a matrix metalloproteinase-2 (MMP2) cleavable polyethylene glycol (PEG)-lipid corona. Near infrared fluorescent dyes and paramagnetic lipids were incorporated to enable dual in vivo optical and magnetic resonance imaging. In the current study we demonstrated that upon incubation with MMP2 the PEG shielding is removed enabling the targeting ligands (RGD peptides) to bind to αvβ3 integrin expressing cells. In vivo mouse imaging revealed that following an intravenous administration our nanoparticles accumulated in the rim of orthotopically implanted breast tumors.

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