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Abstract #1122

13C NMR studies of lymphoma and melanoma cells in the perfusion bioreactor and in vivo xenografts for flux calculation

Seung-Cheol Lee 1 , Jeffrey Roman 1 , Kavindra Nath 1 , David Nelson 1 , Kevin Muriuki 1 , Alexander Shestov 1 , and Jerry Glickson 1

1 Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States

Time course 13C NMR study was performed in the perfused lymphoma and melanoma cells as well as in vivo xenografts for detailed metabolic flux calculation. mTOR signaling inhibitor rapamycin was administered to lymphoma cells and xenografts. Well resolved time course 13C NMR spectra were obtained from both perfused cells and in vivo tumors. mTOR signaling inhibition decreased fluxes to lactate, glutamate as well as glycogen. Melanoma xenografts exhibited higher TCA cycle flux than lymphoma xenografts. Quantitative flux calculation is under process.

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