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Abstract #1123

13C MRS/Bioreactor Technique to Study Melanoma: Quantifying Glutaminolysis and de novo Lipogenesis

Alexander A. Shestov 1 , Anthony Mancuso 2 , Pierre Gilles Henry 3 , Dennis B. Leeper 4 , and Jerry David Glickson 5

1 Radiology, University of Pennsylvania, Philadelphia, PA, United States, 2 Radiology, University of Pennsylvania, PA, United States, 3 University of Minnesota, MN, United States, 4 Radiation Oncology, Thomas Jefferson University, PA, United States, 5 Radiology, Univesity of Pennsylvania, PA, United States

The bioreactor techniques combined with 13C MRS are an important tool to study cancer cell metabolism. Modeling of intracellular MRS isotopomer data obtained during perfusion with 13C labeled substrates allows quantitative determination of transport and metabolic parameters in vivo/in situ. In this work, we apply a novel 13C metabolic flux analysis technique to elucidate cancer metabolism bionetwork and calculate fluxes through important cancer metabolic pathways.

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