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Abstract #1883

Hyperpolarized [2- 13 C]Dihydroxyacetone: Monitoring Hepatic Gluconeogenesis in Real-Time

Karlos X Moreno 1 , Santhosh Satapati 1 , Ralph J DeBerardinis 2 , Shawn C Burgess 1 , Craig R Malloy 1 , and Matthew E Merritt 1

1 Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, United States, 2 Children's Medical Center Research Institute, UT Southwestern Medical Center, Dallas, TX, United States

Direct observation of hyperpolarized glucose from a gluconeogenic substrate has not previously been observed. Isolated mouse livers perfused in a gluconeogenic or glycogenolytic state were injected with hyperpolarized [2- 13 C]dihydroxyacetone. In each condition, hyperpolarized glucose signals were observed. Purified effluent glucose confirmed the enrichment. Minimal differences in hyperpolarized glucose signals between the two conditions were observed. Triose intermediates of glycolysis showed large differences, the glycogenolytic condition having greater signals. This is the first study to demonstrate direct gluconeogenesis using hyperpolarization methods.

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