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Abstract #1906

Brain redox imaging using nitroxide contrast agents in pentylenetetrazol-kindled mice with EPR imaging

Hirotada G Fujii 1 , Miho C Emoto 1 , Mayumi Yamato 2 , and Ken-ichi Yamada 2

1 Center for Medical Education, Sapporo Medical University, Sapporo, Hokkiado, Japan, 2 Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan

Seizure-induced neuronal death is thought to be caused by oxidative stress resulting from generation of reactive oxygen species (ROS). The purpose of this study was to examine the effect of oxidative stress in a pentylenetetrazol (PTZ)-induced seizure mouse model using a redox-sensitive nitroxide, 3-methoxycarbonyl-PROXYL, and to visualize brain redox status by noninvasive EPR imaging. In particular, a remarkable change in redox status in hippocampus of kindled mice was detected by EPR. An in vitro assay showed decreased concentrations of glutathione in the hippocampus after repeated seizures. Both results supported the involvement of ROS generation in epileptic-seizure mouse brain.

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