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Abstract #1917

A Novel Assay for the in vivo Detection of Reactive Oxygen Species Using MRI

Gary Stinnett 1 , Kelly Ann Moore 1 , Errol Loc Samuel 2 , Ming Ge 3 , Brett Graham 3 , James Tour 2 , and Robia G Pautler 1

1 Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States, 2 Department of Chemistry, Rice University, Houston, Texas, United States, 3 Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States

Due to their paramagnetic properties, increased production of superoxide and hydroxyl radicals can cause decreases in T1 relaxation. Animals with increased superoxide and hydroxyl radical production will have lower T1 values in the liver compared to wild type controls. To determine that T1 reduction is due specifically to increased superoxide and hydroxyl radicals, animals were then treated with superoxide scavenging PEG-HCCs. These particles rendered the superoxide and hydroxyl anions inert, increasing T1 values to that of wild type controls. We therefore demonstrate that it is possible to use MRI in conjunction with PEG-HCCs to measure in vivo ROS levels.

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