Optimized Protocol and Evaluation of Referencing Methods in Quantitative 1H NMR Lipid Analysis
Santosh Kumar Bharti 1 and Zaver Bhujwalla 1
Div. of Cancer Imaging Research, The Russell
H. Morgan Dept. of Radiolog and Radiological science,
Johns Hopkins University, School of Medicine, Baltimore,
Maryland, United States
Quantitative lipid analysis helps in understanding role
of lipid in the cancer processes including invasion,
metastasis, proliferation and many other normal and
pathological conditions with clinical interest.
Quantitative NMR analysis of lipids is largely affected
by sample preparation and referencing methods.
Evaporation of TMS during sample preparation
significantly reduces the quantitative accuracy.
Optimized sample preparation protocol and storage showed
improved accuracy and reproducibility. QUANTAS and Stem
co-axial insert containing TSP shows improved qNMR
accuracy over TMS referencing method. QUANTAS offers
easy and rapid quantitative lipids analysis when dealing
with large no of sample and scaling the data for
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