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Abstract #1984

Optimized Protocol and Evaluation of Referencing Methods in Quantitative 1H NMR Lipid Analysis

Santosh Kumar Bharti 1 and Zaver Bhujwalla 1

1 Div. of Cancer Imaging Research, The Russell H. Morgan Dept. of Radiolog and Radiological science, Johns Hopkins University, School of Medicine, Baltimore, Maryland, United States

Quantitative lipid analysis helps in understanding role of lipid in the cancer processes including invasion, metastasis, proliferation and many other normal and pathological conditions with clinical interest. Quantitative NMR analysis of lipids is largely affected by sample preparation and referencing methods. Evaporation of TMS during sample preparation significantly reduces the quantitative accuracy. Optimized sample preparation protocol and storage showed improved accuracy and reproducibility. QUANTAS and Stem co-axial insert containing TSP shows improved qNMR accuracy over TMS referencing method. QUANTAS offers easy and rapid quantitative lipids analysis when dealing with large no of sample and scaling the data for statistical analysis.

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