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Abstract #1987

Direct determination of phosphate sugars in biological material by 1 H High Resolution-Magic Angle Spinning (HR-MAS) NMR spectroscopy

Galle Diserens 1 , Martina Vermathen 2 , Ilche Gjuroski 2 , Sandra Eggimann 3 , Christina Precht 4 , Chris Boesch 1 , and Peter Vermathen 1

1 Depts. Radiology and Clinical Research, University Bern, Bern, Switzerland, 2 Dept. of Chemistry & Biochemistry, University Bern, Bern, Switzerland, 3 University Institute of Clinical Chemistry, Bern University Hospital, Bern, Switzerland, 4 Dept. of Clinical Veterinary Medicine, University Bern, Bern, Switzerland

The study aim was to unambiguously assign nucleotide sugars, mainly UDP-X that are known to be important in glycosylation processes, and glucose-phosphates that are important intermediate metabolites for storage and transfer of energy, directly in spectra of intact cells, as well as in skeletal and heart muscle biopsies by 1 H HR-MAS NMR. A second aim was to study the kinetic of Glc-1P, identified in cardiac muscle. The results demonstrate that sugar phosphates can be determined quickly and non-destructively in cells and biopsies by HR-MAS, which may prove valuable considering the importance of phosphate-sugars in cell metabolism for nucleic acid synthesis.

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