Abstract #1987
Direct determination of phosphate sugars in biological material by 1 H High Resolution-Magic Angle Spinning (HR-MAS) NMR spectroscopy
Galle Diserens 1 , Martina Vermathen 2 , Ilche Gjuroski 2 , Sandra Eggimann 3 , Christina Precht 4 , Chris Boesch 1 , and Peter Vermathen 1
1
Depts. Radiology and Clinical Research,
University Bern, Bern, Switzerland,
2
Dept.
of Chemistry & Biochemistry, University Bern, Bern,
Switzerland,
3
University
Institute of Clinical Chemistry, Bern University
Hospital, Bern, Switzerland,
4
Dept.
of Clinical Veterinary Medicine, University Bern, Bern,
Switzerland
The study aim was to unambiguously assign nucleotide
sugars, mainly UDP-X that are known to be important in
glycosylation processes, and glucose-phosphates that are
important intermediate metabolites for storage and
transfer of energy, directly in spectra of intact cells,
as well as in skeletal and heart muscle biopsies by
1
H
HR-MAS NMR. A second aim was to study the kinetic of
Glc-1P, identified in cardiac muscle. The results
demonstrate that sugar phosphates can be determined
quickly and non-destructively in cells and biopsies by
HR-MAS, which may prove valuable considering the
importance of phosphate-sugars in cell metabolism for
nucleic acid synthesis.
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