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Abstract #0111

Amide proton signals as pH indicator for in vivo MRS and MRI of the brain – Responses to hypercapnia and hypothermia

Takashi Watanabe1, Jens Frahm1, and Thomas Michaelis1

1Biomedizinische NMR Forschungs GmbH, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany

Using proton MRS/MRI of mouse brain at 9.4 T, this work provides the first in vivo evidence of concurrent pH-dependent changes of amide signals and related metabolic responses to hypercapnia and hypothermia. During hypercapnia, amide MRS signals of glutamine and of unspecific compounds increase by ≥50% at 37°C and 22°C. They are strongly correlated with intracellular pH determined from a shift in creatine phosphokinase equilibrium. In MRI, saturation transfer to water protons alters signal intensities in dependence on pH and temperature. Irradiation of aliphatic compounds at -3.5 ppm frequency offset from water predominantly saturates lipids and water associated with myelin.

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