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Abstract #0302

Separation of intracellular and extracellular Z-spectra by DiffusionCEST

Kevin Ray1, Gogulan Karunanithy2, Andrew Baldwin2, Michael Chappell3, and Nicola Sibson1

1Oxford Institute for Radiation Oncology, University of Oxford, Oxford, United Kingdom, 2Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, United Kingdom, 3Institute of Biomedical Engineering, University of Oxford, Oxford, United Kingdom

CEST-MRI is an imaging technique which is sensitive to tissue pH, and has generated pH-weighted images in acute stroke patients. One key assumption regarding CEST-MRI is that the signal is predominantly intracellular. This assumption has implications in the application of CEST-MRI for pH measurement of tumours, which are generally associated with extracellular acidosis. This study developed a novel pulse sequence, combining stimulated echo acquisition mode diffusion and CEST imaging. Using this novel pulse sequence, the intracellular and extracellular contributions to the acquired Z-spectrum were isolated in a simple cell system and post-mortem mouse brain.

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