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Abstract #0596

Assessment of Necrosis in the Ischemic Heart using Hyperpolarized [1,4-13C2]Fumarate

Damian J Tyler1, Angus Lau1, Ferdia Gallagher2, and Marie A Schroeder1

1DPAG, University of Oxford, Oxford, United Kingdom, 2Radiology, University of Cambridge, Cambridge, United Kingdom

The aim of this study was to evaluate the potential of hyperpolarised [1, 4-13C2]fumarate, coupled with MRS, to measure cardiac necrosis during ischemia and reperfusion. Hyperpolarised [1, 4-13C2]fumarate was infused into rat hearts at three time points, corresponding with the healthy heart, early reperfusion after a 20 min ischemic period, and late reperfusion. The amount of [1, 4-13C2]malate production was measured using MRS and quantified to reflect degree of cardiomyocyte necrosis. We observed a 3.8-fold increase in [1,4-13C2]malate during the late reperfusion period but no change in early reperfusion, suggesting that necrotic cell death takes place during reperfusion only. This technique shows potential to evaluate therapies targeting necrosis to prevent cardiac remodeling into failure.

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