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Abstract #0658

Low frequency oscillating gradient spin-echo sequences improves sensitivity to axon diameter – an experimental validation study in live nerve tissue

Lebina Shrestha Kakkar1, Oscar Bennett1, David Atkinson2, Bernard Siow3, James Phillips4, Simon Richardson3, Enrico Kaden1, and Ivana Drobnjak1

1Centre for Medical Image Computing, University College London, London, United Kingdom, 2Centre for Medical Imaging, University College London, London, United Kingdom, 3Centre for Advanced Biomedical Imaging, University College London, London, United Kingdom, 4Department of Biomaterials & Tissue Engineering, University College London, London, United Kingdom

In a recent simulation study, Drobnjak et al demonstrates that low-frequency oscillating gradient spin-echo (OGSE) sequence is more sensitive to axon diameter than conventional pulsed gradient spin-echo (PGSE) sequence when fibre orientation is unknown or when fibre dispersion exists. Here, we experimentally validate this claim. We image a live rat sciatic nerve tissue using both sequences and compare its agreement with histology. Our results confirm that OGSE provides more accurate and precise diameter estimates compared to PGSE. Additionally, OGSE parameter estimates are less affected by reduced number of diffusion gradient directions, suggesting their use could translate into faster scan times.

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