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Abstract #0664

Quantification of anisotropy and directionality in three-dimensional electron microscopy images and diffusion tensor imaging of injured rat brain

Raimo A. Salo1, Ilya Belevich2, Eppu Manninen1, Eija Jokitalo2, Olli Gröhn1, and Alejandra Sierra1

1Department of Neurobiology, A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland, 2Institute of Biotechnology, University of Helsinki, Helsinki, Finland

Diffusion tensor imaging (DTI) is a widely used tool, however, the contribution of brain tissue microstructure into DTI contrast is not fully understood. In this work, we propose using serial block-face scanning electron microscopy (SBEM) and Fourier analysis to gain insight into this contribution. We calculated anisotropy and orientation from SBEM stacks and compare the values to fractional anisotropy and orientation from in vivo and ex vivo DTI. This work will give new insights to the contribution of microstructure to DTI contrast in normal brain and during pathology.

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