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Abstract #0672

Metabolism of hyperpolarized 13C-acetoacetate/β-hydroxybutyrate reveals mitochondrial redox state in perfused rat hearts

Wei Chen1, Chalermchai Khemtong1, Weina Jiang1, Craig R. Malloy1, and A. Dean Sherry1

1Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, United States

A large prior literature on inter-conversion of β-hydroxybutyrate (β-HB) and acetoacetate (AcAc) indicates that the process is mitochondrial and the ratio reflects specifically mitochondrial redox state. Therefore the conversion of [1,3-13C]AcAc to [1,3-13C]β-HB is expected to be sensitive to redox. In this study, we explored the utility of using hyperpolarized [1,3-13C]AcAc to study the mitochondrial redox state in perfused rat hearts. Our results show that the production of HP β-HB from HP-AcAc was much higher in ischemic hearts, reflecting the increased concentration of NADH under this reduced state. The redox-dependent conversion between this metabolic pair in mitochondria may lead to the development of an imaging tool for redox imaging of the heart by hyperpolarized 13C MRI.

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