Abstract #0672

Metabolism of hyperpolarized 13C-acetoacetate/β-hydroxybutyrate reveals mitochondrial redox state in perfused rat hearts

Wei Chen¹, Chalermchai Khemtong¹, Weina Jiang¹, Craig R. Malloy¹, and A. Dean Sherry¹

¹Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, United States

A large prior literature on inter-conversion of β-hydroxybutyrate (β-HB) and acetoacetate (AcAc) indicates that the process is mitochondrial and the ratio reflects specifically mitochondrial redox state. Therefore the conversion of [1,3-¹³C]AcAc to [1,3-¹³C]β-HB is expected to be sensitive to redox. In this study, we explored the utility of using hyperpolarized [1,3-¹³C]AcAc to study the mitochondrial redox state in perfused rat hearts. Our results show that the production of HP β-HB from HP-AcAc was much higher in ischemic hearts, reflecting the increased concentration of NADH under this reduced state. The redox-dependent conversion between this metabolic pair in mitochondria may lead to the development of an imaging tool for redox imaging of the heart by hyperpolarized ¹³C MRI.

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