Abstract #0761

Validation and Optimization of Calibrated fMRI from oxygen-sensitive Two-Photon Microscopy of the mouse brain

Louis Gagnon^1,2,3, Sava Sakadzic⁴, Frederic Lesage², Philippe Pouliot², Anders M Dale⁵, Anna Devor⁵, Richard B Buxton⁵, and David A Boas³

¹Department of Medicine, Laval University, Quebec, QC, Canada, ²Department of Electrical Engineering, Ecole Polytechnique Montreal, Montreal, QC, Canada, ³Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States, ⁴Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Chalestown, MA, United States, ⁵Department of Radiology and Neuroscience, UCSD, La Jolla, CA, United States

Calibrated fMRI allows to estimate relative changes in the Cerebral Metabolic Rate of Oxygen Consumption (rCMRO₂) from combined BOLD and ASL measurements during a functional task. Here, we improved the accuracy of the approach by using Two-Photon microscopic measurements of the cortical microvasculature together with first principle Monte Carlo simulations of proton diffusion across the two-photon volumes. Our method allowed (1) to validate Calibrated fMRI from the microscopic point of view and (2) to optimize the free parameters of the biophysical model assumed, therefore increasing the accuracy of this method to estimate rCMRO₂.

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