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Abstract #0838

NMR metabolomics of biofluids for early diagnosis of brain metastasis

James R Larkin1, Alex M Dickens2, Timothy D W Claridge3, Daniel C Anthony2, and Nicola R Sibson1

1CRUK and MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, United Kingdom, 2Department of Pharmacology, University of Oxford, Oxford, United Kingdom, 3Department of Chemistry, University of Oxford, Oxford, United Kingdom

Secondary tumours, or metastases, in the brain are currently detected at a late stage by gadolinium-enhanced MRI. We used mouse models of brain metastases, coupled with high resolution NMR of urine to identify characteristic patterns of metabolites in tumour-bearing animals. A model with a tumour cells implanted directly in the brain showed sensitive and specific detection at day 5 with increasing separation at later time points. Models injecting cells into the heart or venous circulation give rise to differing systemic and central nervous system (CNS) tumour burdens. Metabolite patterns allow identification of these animals with a heavy CNS tumour burden.

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