Abstract #1022

Direct Quantitative 13C-Filtered 1H Magnetic Resonance Imaging of Pegylated Biomacromolecules In Vivo

Rohan Alvares¹, Justin Lau^2,3, Peter Macdonald¹, Charles Cunningham^2,3, and R. Scott Prosser¹

¹Department of Chemistry, University of Toronto, Toronto, ON, Canada, ²Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada, ³Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada

We demonstrate a new platform technology in which macromolecular constituents, such as proteins and drug delivery systems, are observed directly and quantitatively in vivo using ¹H MRI of ¹³C-labeled polyethylene glycol (¹³C-PEG) tags. The 28 kDa ¹³C-PEG tags are non-immunogenic, and each bears approximately 2500 spectroscopically equivalent ¹H nuclei appearing at a single resonance position. By filtering the ¹H PEG signal through the directly coupled ¹³C nuclei, background water and fat signals are largely eliminated. We demonstrate the approach by monitoring in real-time the distribution of ¹³C-PEG and ¹³C-pegylated albumin injected into the hind leg of a mouse.

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