We investigated the dependencies of the exchange-relayed Nuclear Overhouser Effect (rNOE) observable in Chemical Exchange Saturation Transfer (CEST) experiments on tissue integrity and static magnetic field strength B0. By comparison of a homogenized and native sample of white matter tissue of animal brain we could show that the CEST signal of the aliphatic rNOE is independent of tissue structure. The observed increase of all CEST effects on decrease of B0 probably results from relatively broader saturation bandwidth at lower field strengths. No indication for a rNOE dependency on B0 differing from that of chemical exchange effects could be found.
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