Interscapular brown adipose tissue plays an important role in the maintenance of core body temperature of small mammals through a process known as nonshivering thermogenesis. A dense vascular system delivers oxygen for the thermogenesis and is needed for the transport of produced heat from iBAT towards thoracic and abdominal areas. Therefore iBAT blood perfusion is an important parameter for analyzing iBAT activation and function.
The FAIR ASL protocol developed within this study accounts for the high lipid content in the interscapular area as well as the structure of the iBAT vascular system.iBAT blood perfusion was determined in wildtype mice and the used protocol is justified based on perfusion numbers determined from skeletal muscle.
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