Meeting Banner
Abstract #2010

A quantitative measurement of the cell membrane water permeability of expression-controlled AQP4 cells with diffusion weighted MRI

Takayuki Obata1, Jeff Kershaw2, Yasuhiko Tachibana1, Youichiro Abe3, Sayaka Shibata2, Yoko Ikoma2, Hiroshi Kawaguchi4, Ichio Aoki2, and Masato Yasui3

1Applied MRI Research, National Institute of Radiological Sciences, Chiba, Japan, 2Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan, 3Department of Pharmacology, Keio University, Tokyo, Japan, 4Human Informatics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

We performed multi-b and multi-diffusion-time DWI on aquaporin-4-expressing and non-expressing cells, and demonstrated a clear difference between the signals from the two cell types. The data was interpreted with a two-compartment model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange times with this model were comparable with those measured using an independent optical imaging technique, which suggests that this method might be used to characterize cell-membrane water permeability. As the technique can be applied in routine clinical examination, it has the potential to improve clinical diagnosis.

This abstract and the presentation materials are available to members only; a login is required.

Join Here