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Abstract #2304

Fluorescence-based Quantification of Gadolinium-bound Liposomes using Carbostyril 124-sensitized DTPA

Lindsay Kathleen Hill1,2, Stewart Russell3,4, Dung Minh Hoang1, and Youssef Zaim Wadghiri1

1Radiology, NYU School of Medicine, New York, NY, United States, 2Biomedical Engineering, SUNY Downstate Medical Center, Brooklyn, NY, United States, 3Thayer School of Engineering, Dartmouth College, Hanover, NH, United States, 4Department of Mechanical Engineering, The City College of New York, New York, NY, United States

Advancement in the field of Gadolinium-bound contrast agent discovery is reliant on the development and characterization of novel constructs made in-house. However, the assessment of physicochemical properties and in vivo pharmacokinetics, requiring highly sensitive measurements, is often impeded by the lack of analytical techniques that are simultaneously sensitive, affordable, and accessible. Here we demonstrate that Carbostyril 124-sensitized DTPA can be incorporated into a lipid-based microparticle, allowing for rapid quantification of Gadolinium concentration with nanomolar sensitivity using a readily available fluorescence plate reader. This sensitive and convenient technique could rapidly propel the characterization of novel MR contrast agents.

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