Abstract #2332

The formulation of hyperpolarized 13C pyruvate solutions influences the labeling of myocardial metabolites in vivo

Hikari A. I. Yoshihara¹, Jessica A. M. Bastiaansen², Corinne Berthonneche³, Arnaud Comment¹, and Juerg Schwitter⁴

¹Institute of Physics of Biological Systems, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland, ²Department of Radiology, University Hospital Lausanne (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland, ³Cardiovascular Assessment Facility, University Hospital Lausanne (CHUV), Lausanne, Switzerland, ⁴Division of Cardiology and Cardiac MR Center, University Hospital Lausanne (CHUV), Lausanne, Switzerland

In developing an intact rat model for myocardial ischemia using hyperpolarized ¹³C pyruvate, different compound formulations were evaluated. Infusion of 4-hydroxy-TEMPO-polarized sodium [1-¹³C]pyruvate was compared to an equivalent dose of buffered trityl radical-polarized [1-¹³C]pyruvic acid. Whereas higher levels of polarization and MRS signal were obtained with trityl radical, the metabolite signals normalized to total signal were lower. In particular, [1-¹³C]lactate signal relative to total signal was markedly higher using TEMPO-polarized pyruvate. [¹³C]bicarbonate and [1-¹³C]alanine signals were affected to a lesser degree. This study demonstrates the composition of the infused hyperpolarized pyruvate solution can significantly affect its metabolism in vivo.

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