Abstract #2346

Feasibility of probing lactate metabolism and neuroprotection in a mouse model of stroke using hyperpolarized 13C-lactate

Mor Mishkovsky¹, Lara Buscemi², Ximena Castillo², Mario Lepore³, Arnaud Comment⁴, Lorenz Hirt², and Jean-Noël Hyacinthe^5,6

¹Laboratory of Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland, ²Department of Clinical Neurosciences, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland, ³Centre d'Imagerie Biomédicale (CIBM), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland, ⁴Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland, ⁵School of Health Sciences - Geneva, University of Applied Sciences and Arts Western Switzerland, Geneva, Switzerland, ⁶Image Guided Intervention Laboratory, University of Geneva, Geneva, Switzerland

Stroke is a major public health challenge in the context of the current demographic changes. Among a wide range of applications, hyperpolarized magnetic resonance enables in vivo real-time measurement of biochemical transformations of hyperpolarized ¹³C-labeled precursors, including lactate, a known neuroprotectant in stroke at the preclinical level. This study shows the feasibility of measuring lactate metabolism in vivo in a mouse model of stroke (MCAO) following intravenous injection of hyperpolarized L-[1-¹³C]lactate. Calculated pyruvate-to-lactate ratio shows an increased labeling of the pyruvate pool in MCAO when compared to sham. This feasibility study suggests new perspectives to understand lactate biodistribution and its neuroprotective effect in stroke.

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